Advanced Chromatography in Therapeutic Oligonucleotide Analysis

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This lecture provides a comprehensive overview of advanced chromatographic techniques vital for oligonucleotide analysis. We will explore the fundamentals of chromatography, examining the roles of ion-exchange, RP-IP, and 2D-LC methods in impurity separation. Through detailed case studies, such as comparing AEX to RP-IP and improvements via phenyl columns, attendees will gain insights into validated IP-HPLC-MS methods. Concluding with key findings and future research directions, this session...