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This research investigates Ala-(1-5), a novel peptide within the renin-angiotensin system (RAS), focusing on its formation, cardiovascular effects, and receptor interactions. Using methods like mass spectrometry, experiments with SHR rats, CHO cells, and receptor-deficient mice revealed ACE-driven Ala-(1-5) synthesis, diverse vascular and cardiac impacts, and central effects on hypertension. The findings highlight its potential as a triple receptor agonist with implications for hypertension...