Presentation on components,detailed mechanism, advantage-disadvantage, applicationand limitations of PacBio sequencing

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PacBio sequencing is a revolutionary long-read technology that addresses the limitations of traditional methods like Sanger and short-read sequencing. By utilizing single-molecule real-time (SMRT) sequencing, it enables comprehensive analysis of complex genomes. Key components of a PacBio system include the DNA template, SMRTbell circular DNA construct, and immobilized DNA polymerase in a zero-mode waveguide (ZMW), which together enhance the accuracy and depth of genomic insights.