Designing and Validating Optogenetic Constructs using SSRs

This research focuses on utilizing site-specific recombinases (SSRs) for optogenetic control in genetic circuits. Key aspects include modular plasmid design, cloning workflows using advanced Gibson Assembly, and iterative refinement processes to address challenges. Validation methods highlight successful activation outcomes, such as red light-induced GFP fluorescence, showcasing functional results. The study emphasizes the implications for synthetic biology, promising applications in...