Mapping Transcriptional Regulation of Bacterial Persistence

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This lecture provides a comprehensive exploration of "Regulatory Time-Kill Sequencing (RTK-seq)" within the framework of a doctoral thesis presentation. The session begins with an introduction to RTK-seq and its underpinning technologies, such as CRISPRi and barcode sequencing. It progresses through key findings, showcasing workflow dynamics, survival curve analyses, and persistence phenotypes. Validation processes, including RT-qPCR, will be discussed, alongside the identification of...