Protoplasts and Somatic Hybridization: Methods and Applications

This theory-focused overview explores protoplasts, covering their definition, historical production methods, and applications. It details isolation techniques, emphasizing enzymatic methods and osmotic stabilizers. Efficient culturing is examined with viability assessments and ideal medium composition. Somatic hybridization is analyzed, including types, examples, and trait transfers, alongside challenges such as infertility, genetic instability, and selection hurdles.