Innovative RNA-Guided Nucleases for Genome Editing

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This study introduces minimal RNA-guided nucleases, focusing on TnpBs design strategies through structure-guided inverse folding, evolutionary residue constraints, and protein-RNA/DNA contact strategies. High-throughput screening revealed enhanced activity in new variants. Mechanistic insights through Cryo-EM of variant v7 uncovered novel RNA/DNA contacts and unresolved TAM-bound intermediates. The research synthesizes significant findings and their broader implications, highlighting...