go through the paper, summarize all the chemistry including the linker chemistry, photochemistry and click chemistry with the concepts how Circular ASO advance the field and provide a full document containing the summary. Also prepare the pwer point slide with detailed notes for this talk. Consider me a biology person who don't understand chemistry and I need detailed explanation for this paper to give the talk Near-Infrared Light-Activated Circular Oligonucleotides for Spatiotemporal Control of Gene Silencing in Mice Rong Wang+, Wen Chen+, Qiu Hong Qu, Junjie Liu, Hang Xing, Jian-Hui Jiang,* and Zhenkun Wu*
go through the paper, summarize all the chemistry including the linker chemistry, photochemistry and click chemistry with the concepts how Circular ASO advance the field and provide a full document containing the summary. Also prepare the pwer point slide with detailed notes for this talk. Consider me a biology person who don't understand chemistry and I need detailed explanation for this paper to give the talk Near-Infrared Light-Activated Circular Oligonucleotides for Spatiotemporal Control of Gene Silencing in Mice Rong Wang+, Wen Chen+, Qiu Hong Qu, Junjie Liu, Hang Xing, Jian-Hui Jiang,* and Zhenkun Wu*
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This research explores the transition from chemical switches to circular oligonucleotides, addressing the risks of uncontrolled gene silencing. It presents evidence of effective reactivation and gene silencing through DNAzyme 10-23, achieving a 78% yield in circularization, confirmed by PAGE, HPLC, and MS. The study highlights the spatial control of tumors using MCF-7 xenografts, demonstrating that LNP signals appear in tumors within 2 hours and persist for at least 24 hours, indicating...