Understanding High-Performance Liquid Chromatography (HPLC)

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This overview covers the principles and classification of High-Performance Liquid Chromatography (HPLC), detailing its uses and advantages. It explains the separation principle involving stationary and mobile phases, alongside HPLC theory. The instrumentation section outlines key components such as the solvent reservoir, pump, and detector, while method development focuses on optimizing conditions for analytes. Finally, it addresses validation and analytical performance, emphasizing factors...