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This study focuses on DNA methylation's role in oral squamous cell carcinoma, emphasising early diagnosis. Objectives include assessing methylation patterns and lifestyle factor impacts, supported by cross-sectional analysis. Mechanisms like MGMT and P16 promoter hypermethylation in cancer are reviewed. The methodology involves defined sample sizes, analytical processes, and statistical testing under ethical guidelines. Approvals and confidentiality are ensured, aiming to uncover...