Simultaneous Avian Influenza Detection with TLAMP

This presentation outlines a novel TLAMP assay design targeting influenza subtypes H5, H7, and H9 by integrating LAMP with fluorescent probes. Optimized primer ratios, reagents, and the Bst 2.0 enzyme enhance sensitivity and specificity while reducing contamination risks. Clinical evaluations demonstrate 100% sample consistency and highlight the assay’s potential for point-of-care use in rural labs, supporting effective outbreak control and informed health decisions.