Harnessing Ketoreductases for Sustainable Chemistry

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This project explores ketoreductases, enzymes that convert ketones into chiral alcohols, highlighting their role in sustainable chemistry. It covers the cloning, expression, and purification of KpKR in Komagataella phaffii, comparing various vectors and purification methods. Additionally, the project investigates KpKR's activity with prochiral ketones, optimising conditions such as pH and substrate concentration, while employing advanced techniques like ribosome display and computational...