Create around 10-15 slides for a discussion class to cover genome editing. these slides would serve as a outline of the whole field. contexts include but not limited to (1) why do we do genome editing; (2) genome editing methods before CRISPR-Cas; (3) CRISPR-Cas based gene disruption; (4) base editor; (5) prime editing; (6) transposes based gene integration; (7) recombinase based gene integration. cite the key research reference for each technology. there will be several following classes where students will present the key research paper and discuss about the key discovery. student will learn by reading the history and the cutting edge technologies and via discussion. include one slide to emphasize the teaching philosophy of this course. students' scores would be determined by participation on class and reading before class, and presentation. include this evaluation rule in one slide.