Calibration Curve Selection in Immunoassays

This presentation explores immunoassay calibration, emphasizing its definition, significance, and key methods. It delves into the application of calibration curves in analyte quantitation and clinical diagnostics, comparing sigmoidal and log-linear models for accuracy and dynamic range. Challenges like the high-dose hook effect and signal saturation are discussed alongside strategies for mitigation. Practical examples, like hormone analysis protocols, highlight real-world relevance. Quality...