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This study investigates the antibacterial efficacy of NFH9 against three bacterial strains through a structured experimental workflow. It includes antibacterial assays such as inhibition zone diameter (IZD) and minimum inhibitory concentration (MIC), alongside mechanistic evaluations like killing rates and biofilm suppression. NFH9 demonstrates significant antibacterial activity, with MICs between 8-16 µg/mL, and shows a concentration-dependent bactericidal effect, achieving 75.35% cell...