Optimizing Metal-Enhanced Fluorescence for Biomarker Detection

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This presentation delves into the Metal-Enhanced Fluorescence (MEF) Immunoassay, highlighting the role of Cu/Au plasmonic beads. It explores the mechanism of surface plasmon resonance and its enhancement of fluorescence in biomarker detection. The assay's components, including the use of self-assembled monolayers and Alexa Fluor 647, are examined. We discuss optimization techniques, focusing on surface cleaning and antibody immobilization. Performance metrics are presented to evaluate...